From the study of the remarkable adhesion of mussel to various type of surfaces under harsh marine conditions, the presence in high content of catechol-containing 3,4 dihydrophenylalanine in the secreted adhesive foot protein was established. Since this discovery, an ever-increasing number of bio-inspired catechol-based polymers have been exploited for the fabrication of advanced materials such as water-resistant or biomedical adhesives, multifunctional coatings for biomedical or energy applications. Up to date, catechol and/or quinone functionalized layers have been synthesized from multi-step wet chemical techniques presenting low deposition rates. Recently, a novel and promising one-step deposition route has been identified at LIST using an aerosol-assisted atmospheric-pressure plasma polymerization method (European patent).The main motivation of the BIOREAFILM project will be to develop an aerosol-assisted atmospheric-pressure plasma copolymerization method to synthesize adherent and resistant layers presenting tuneable amount of catechol or/and quinone groups with high deposition rates. With this aim, a complete study of the layer growth mechanism will be realized based on the development of in-situ physical and chemical measurements (OES, GC/MS) and of novel analytical methodologies for estimating the catechol/quinone surface density and repartition in the deposited film. These results will be correlated to the chemical and physical properties of the films determined by complementary techniques (UV, IR, XPS, ToF-SIMS, resistance tests).At the end of the project, it is planned to synthesis stable layers presenting a compositional gradient in catechol-quinone groups and to demonstrate its performance for the immobilization of biomolecules. The innovative BIOREAFILM approach, combined with the biomolecular engineering, is expected to open the way for future developments in the field of new advanced bioactive surfaces.