CONFINED NANOSCALE GEOMETRIES TO ENHANCE SENSITIVITY OF PLASMONIC IMMUNOASSAYS

SCHEME: CORE

CALL: 2015

DOMAIN: MS - New Functional and Intelligent Materials and Surfaces

FIRST NAME: Sivashankar

LAST NAME: Krishnamoorthy

INDUSTRY PARTNERSHIP / PPP: No

INDUSTRY / PPP PARTNER:

HOST INSTITUTION: LIST

KEYWORDS: Nanoarrays; Plasmonic Sensing; Structure-Activity Relationships; Localised surface plasmon resonance (LSPR); Surface Enhanced Raman Spectroscopy (SERS) and Metal-Enhanced Fluorescence (MEF);

START: 2016-09-01

END: 2019-08-31

WEBSITE: https://www.list.lu/

Submitted Abstract

Sensitive transduction of bio-molecular binding events on chip carries profound implications to the outcome of a range of in vitro sensors.This includes biosensors that address research as well as diagnostic questions of clinical relevance, e.g. profiling of biomarkers, protein expression, drug and toxicity screening, drug-efficacy monitoring, among others. Nanostructured biosensors constitute a promising advance in this direction owing to their ability in catering to better sensitivity, response times, and miniaturization, in addition to imparting smart, intelligent capabilities to interfaces. Amongst nanoscale sensors, plasmonic biosensing involving electromagnetic (EM) near-field enhancements for detection and quantification of molecules relies critically on control over nanoscale geometries in noble metal structures at ultra-high (or molecular) resolutions. At these resolutions the control over geometric attributes translates into an advantage in controlling near-field profile and intensity of EM field. This can be favorably taken advantage towards highly sensitive transduction of molecular binding events through surface-enhanced spectroscopies such as surface-enhanced Raman spectroscopy (SERS), metal enhanced Fluorescence (MEF) and localized surface plasmon resonance imaging (LSPR). These geometries typically exploit structural attributes such as nanoscale curvatures or gaps in metal structures spanning a few nanometers, thus with length scales that typically overlap with the size of small proteins. Such geometries invariably introduces constraints on the molecular binding response, thus altering the interaction outcomes, viz. density and kinetics of adsorption, molecular orientations, in a manner that would impact the resulting optical response. A careful engineering of the nanoscale geometries can simultaneously take advantage of EM field enhancements together with molecular interaction within nanoscale geometries. To this end, PLASENS aims at an engineered nanoscale interface with geometry tailored to simultaneously favour molecular adsorption and plasmonic enhancements, with optimal geometries drawn from structure-response and structure-optical property correlations. PLASENS consortium employs experiments in feedback with computational simulations of optical and biomolecular response on nanoplasmonic interfaces to enable rational design rules in fabrication. PLASENS is a constituted by a multidisciplinary and international team of researchers from the domains of surface chemistry, nano optics, nanospectroscopy, biomedicine, and theoretical/computational simulations. The successful outcome of PLASENS carries implications beyond the delivery of fundamental scientific knowledge in the design of plasmonic interfaces, into technologically attractive interfaces with practical biomedical relevance.

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