DNADIVE aims at developing a toolbox for the molecular monitoring of invasive crayfish in the streams of Luxembourg. Three exotic species (Orconectes limosus, Pacifastacus leniusculus and Astacus leptodactylus) and a native one (Astacus astacus) will be targeted for the project. This molecular toolbox will encompass several techniques of detection comprising (1) a simple amplification method easily performed in a laboratory with few elements, (2) a digital droplet amplification (ddPCR) which is a more elaborated lab method that can allow for a higher detection sensitivity and a possible quantification of DNA that could be related through the proxy of biomass and abundance to the size of the populations detected and (3) an isotherm amplification method (iPCR) i.e. a simple, cost effective approach which will allow for a field detection usable by non-trained agents. The results will enable the development of a predictive species distribution model for the target species and to infer their impact on freshwater communities through the comparison with previous sampling campaigns.This set of methods has the high potential to efficiently contribute to early detection and routine monitoring of the invasive crayfish species in Luxembourg, thus allowing for a timely and efficient decision-making and appropriate management.